Common name: Sabouraud glucose agar medium
English name:Sabouraud Dextrose Agar
Used for the cultivation and counting of molds and yeast.
Culture medium is a mixed nutritional product prepared by artificial methods, specifically for microbial cultivation, separation, identification, research, and preservation. A mixture of animal tissue pepsin hydrolysate and tryptone provides nitrogen sources, vitamins, and growth factors; Glucose provides a carbon source; Agar is a coagulant.
It is composed of a mixture of animal tissue pepsin hydrolysate and tryptone, as well as culture medium materials such as glucose and agar, which are milled and packaged.
After pre-treatment according to the pharmacopoeia standards, the samples were inoculated into the culture medium and incubated at 20-25 ℃ for 24-48 hours or 5-7 days, and the results were observed.
Aspergillus niger grows well, with white hyphae and black spores; Candida albicans grow well, with milky white colonies that are smooth.
1. This culture medium is only used for scientific research.
2. The collected specimens must be inoculated and cultured as soon as possible to ensure accurate results.
3. The specimens used and their cultivation waste should be considered as potentially infectious substances and should be disposed of in accordance with the operating standards of infectious disease laboratories.
4~30 ℃, stored away from light.
【Period of validity】
The validity period is 3 years.
1. Chinese Pharmacopoeia 2020 Edition
2. SN/T 1538.1-2005 Guidelines for the Preparation of Culture Medium Part 1: General Principles for Quality Assurance of Laboratory Culture Medium Preparation
3. SN/T 1538.2-2007 Guidelines for the Preparation of Culture Media Part 2: Practical Guidelines for Performance Testing of Culture Media
4. WS/T 232-2002 Quality Inspection Procedures for Commercial Microbial Culture Media
5. GB 4789.28-2013 National Food Safety Standard - Quality Requirements for Food Microbiology Testing Media and Reagents