Common name: Columbia blood agar medium
English name:Columbia Blood Agar
Φ 90mm, 10 dishes/bag.
Used for the cultivation and hemolysis test of bacteria with high nutritional requirements, as well as for the detection of Clostridium in drugs.
Tryptone, trypsin digest, stomach enzyme digest, yeast extract powder, soluble starch provide carbon and nitrogen sources, vitamins, and growth factors; Sodium chloride can maintain a balanced osmotic pressure; Agar is a coagulant.
It is composed of tryptone, trypsin digest, stomach enzyme digest, yeast extract powder, soluble starch, sodium chloride, and agar.
After pre-treatment according to pharmaceutical industry standards, the samples were inoculated into culture medium and incubated at 36 ± 1 ℃ for 18-24 hours or 24-48 hours, and the results were observed.
Staphylococcus aureus and beta hemolytic streptococcus grew well, β hemolysis; streptococcus pneumoniae grows well, α hemolysis; escherichia coli grows well without hemolysis.
1. This culture medium is only used for scientific research.
2. The collected specimens must be inoculated and cultured as soon as possible to ensure accurate results.
3. The specimens used and their cultivation waste should be considered as potentially infectious substances and should be disposed of in accordance with the operating standards of infectious disease laboratories.
Store at 2-8 ℃ away from light.
【Period of validity】
The validity period is 3 months.
1. YY/T 0576-2005 Colombian Blood Agar Basic Culture Medium, Pharmaceutical Industry Standard of the People's Republic of China for Migrant Workers
2. SN/T 1538.1-2005 Guidelines for the Preparation of Culture Medium Part 1: General Principles for Quality Assurance of Laboratory Culture Medium Preparation
3. SN/T 1538.2-2007 Guidelines for the Preparation of Culture Media Part 2: Practical Guidelines for Performance Testing of Culture Media
4. WS/T 232-2002 Quality Inspection Procedures for Commercial Microbial Culture Media
5. GB 4789.28-2013 National Food Safety Standard - Quality Requirements for Food Microbiology Testing Media and Reagents