Common Name: MacConkey Agar (MAC)
English name:Maconkey Agar
Used for selective isolation and cultivation of intestinal pathogenic bacteria.
Culture medium is a mixed nutritional product prepared by artificial methods, specifically for microbial cultivation, separation, identification, research, and preservation. The peptone contained in MacConkey agar (MAC) provides carbon and nitrogen sources, vitamins, and growth factors; Lactose is a fermentable sugar; Sodium chloride maintains intracellular and extracellular osmotic pressure; Bile salt and crystal violet can inhibit the growth of Gram positive bacteria; Neutral red is a pH indicator.
It is made from culture medium materials such as peptone, lactose, sodium chloride, bile salt, crystal violet agar, and indicator by ball milling and packaging.
After pre-treatment according to GB standards, the samples were inoculated into a culture medium at 36 ± 1 ℃ for 24-48 hours, and the results were observed.
Shigella flexneri grows well, colorless to light pink, semi transparent brown or green colonies; Escherichia coli grows well, bright peach red or pink, and can have bile acid precipitation; The growth of Staphylococcus aureus is inhibited.
1. This culture medium is only used for scientific research.
2. The collected specimens must be inoculated and cultured as soon as possible to ensure accurate results.
3. The specimens used and their cultivation waste should be considered as potentially infectious substances and should be disposed of in accordance with the operating standards of infectious disease laboratories.
4-30 ℃, stored away from light.
【Period of validity】
The validity period is 3 years.
1. GB 4789.6-2016 National Food Safety Standard - Microbiological Examination of Food - Examination of Escherichia coli causing diarrhea
2. SN/T 1538.1-2005 Guidelines for the Preparation of Culture Medium Part 1: General Principles for Quality Assurance of Laboratory Culture Medium Preparation
3. SN/T 1538.2-2007 Guidelines for the Preparation of Culture Media Part 2: Practical Guidelines for Performance Testing of Culture Media
4. WS/T 232-2002 Quality Inspection Procedures for Commercial Microbial Culture Media
5. GB 4789.28-2013 Quality Requirements for Culture Media and Reagents for Microbiological Testing of Food