DMEM is a widely used basal cell culture medium obtained from Dulbecco modified Eagle medium, suitable for various mammalian cell cultures, including fibroblasts, neurons, glial cells, smooth muscle cells, as well as fine cell lines such as HeLa, 293, Cos-7, and PC-12.
DMEM is a cell culture medium containing various amino acids and glucose, developed on the basis of MEM cell culture medium. Compared with MEM, the dosage of various components has been increased. DMEM high sugar type has advantages such as facilitating cell adhesion, being suitable for fast growth, high-density culture, etc; Low sugar type is more suitable for the cultivation of cells with slower metabolism and low glucose demand.
This type of culture medium is widely used in vaccine production, cell culture of various primary virus host cells, and single cell culture, such as cell lines A9, 3T6, BALB/3T3, COS-1, COS-3, COS-7, L6, WEHI-3b, etc.
1. The difference between DMEM and other culture media is that it contains four times the concentration of amino acids and vitamins equivalent to the initial Eagle essential culture medium, which is rich in nutrients and has strong caching ability. Adopting double HCO3- and CO2 concentrations provides better buffering effect;
2. Wide applicability: DMEM high sugar culture medium is widely used for cells with fast growth and low adhesion, or high-density suspension cell culture;
3. Sterile products 0.22 μ M filter membrane filtration for sterilization, without the need for high-temperature and high-pressure sterilization, with minimal nutrient loss and ready to use upon opening the bottle.